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jurkat dcas9 krab cell lines  (Addgene inc)


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    Addgene inc jurkat dcas9 krab cell lines
    Jurkat Dcas9 Krab Cell Lines, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 354 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+lenti+dcas+krab+blast/lenti+dCAS-VP64_Blast+(Plasmid+%2361425)/pmc12757029-489-4-12
    Average 96 stars, based on 354 article reviews
    jurkat dcas9 krab cell lines - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Targeted long-read sequencing of the Ewing sarcoma 6p25.1 susceptibility locus identifies germline-somatic interactions with EWSR1-FLI1 binding.
    Article Snippet: .. The A673 dCas9-KRAB-expressing cell line was created as described previously12; the A673 cell line was lentivirally transduced using the plasmid Lenti-dCas-KRAB-blast (Addgene) with a MOI of 3, cell selection was performed using 20 mg/mL blasticidin. siRNA transfection For qPCR knockdown validation following siRNA transfection, 75,000 A673 cells were seeded in each well of a 6-well plate. .. The following day, individual siRNAs against RREB1 (Dharmacon ON-TARGETplus Human RREB1 siRNA J-019150-05-0005, J-019150-07-0005, and J-019150-08-0005, referred to as siRREB1 #1, #3, and #4, respectively) (Dharmacon/Horizon Discovery), and control siRNA (siCT, Dharmacon/Horizon Discovery) were transfected using lipofectamine RNAiMAX (Thermo Fisher/ Invitrogen) (50 nM siRNA, lipofectamine ratio 1), using manufacturers protocols.

    Selection:

    Article Title: Targeted long-read sequencing of the Ewing sarcoma 6p25.1 susceptibility locus identifies germline-somatic interactions with EWSR1-FLI1 binding.
    Article Snippet: .. The A673 dCas9-KRAB-expressing cell line was created as described previously12; the A673 cell line was lentivirally transduced using the plasmid Lenti-dCas-KRAB-blast (Addgene) with a MOI of 3, cell selection was performed using 20 mg/mL blasticidin. siRNA transfection For qPCR knockdown validation following siRNA transfection, 75,000 A673 cells were seeded in each well of a 6-well plate. .. The following day, individual siRNAs against RREB1 (Dharmacon ON-TARGETplus Human RREB1 siRNA J-019150-05-0005, J-019150-07-0005, and J-019150-08-0005, referred to as siRREB1 #1, #3, and #4, respectively) (Dharmacon/Horizon Discovery), and control siRNA (siCT, Dharmacon/Horizon Discovery) were transfected using lipofectamine RNAiMAX (Thermo Fisher/ Invitrogen) (50 nM siRNA, lipofectamine ratio 1), using manufacturers protocols.

    Transfection:

    Article Title: Targeted long-read sequencing of the Ewing sarcoma 6p25.1 susceptibility locus identifies germline-somatic interactions with EWSR1-FLI1 binding.
    Article Snippet: .. The A673 dCas9-KRAB-expressing cell line was created as described previously12; the A673 cell line was lentivirally transduced using the plasmid Lenti-dCas-KRAB-blast (Addgene) with a MOI of 3, cell selection was performed using 20 mg/mL blasticidin. siRNA transfection For qPCR knockdown validation following siRNA transfection, 75,000 A673 cells were seeded in each well of a 6-well plate. .. The following day, individual siRNAs against RREB1 (Dharmacon ON-TARGETplus Human RREB1 siRNA J-019150-05-0005, J-019150-07-0005, and J-019150-08-0005, referred to as siRREB1 #1, #3, and #4, respectively) (Dharmacon/Horizon Discovery), and control siRNA (siCT, Dharmacon/Horizon Discovery) were transfected using lipofectamine RNAiMAX (Thermo Fisher/ Invitrogen) (50 nM siRNA, lipofectamine ratio 1), using manufacturers protocols.

    Real-time Polymerase Chain Reaction:

    Article Title: Targeted long-read sequencing of the Ewing sarcoma 6p25.1 susceptibility locus identifies germline-somatic interactions with EWSR1-FLI1 binding.
    Article Snippet: .. The A673 dCas9-KRAB-expressing cell line was created as described previously12; the A673 cell line was lentivirally transduced using the plasmid Lenti-dCas-KRAB-blast (Addgene) with a MOI of 3, cell selection was performed using 20 mg/mL blasticidin. siRNA transfection For qPCR knockdown validation following siRNA transfection, 75,000 A673 cells were seeded in each well of a 6-well plate. .. The following day, individual siRNAs against RREB1 (Dharmacon ON-TARGETplus Human RREB1 siRNA J-019150-05-0005, J-019150-07-0005, and J-019150-08-0005, referred to as siRREB1 #1, #3, and #4, respectively) (Dharmacon/Horizon Discovery), and control siRNA (siCT, Dharmacon/Horizon Discovery) were transfected using lipofectamine RNAiMAX (Thermo Fisher/ Invitrogen) (50 nM siRNA, lipofectamine ratio 1), using manufacturers protocols.

    Knockdown:

    Article Title: Targeted long-read sequencing of the Ewing sarcoma 6p25.1 susceptibility locus identifies germline-somatic interactions with EWSR1-FLI1 binding.
    Article Snippet: .. The A673 dCas9-KRAB-expressing cell line was created as described previously12; the A673 cell line was lentivirally transduced using the plasmid Lenti-dCas-KRAB-blast (Addgene) with a MOI of 3, cell selection was performed using 20 mg/mL blasticidin. siRNA transfection For qPCR knockdown validation following siRNA transfection, 75,000 A673 cells were seeded in each well of a 6-well plate. .. The following day, individual siRNAs against RREB1 (Dharmacon ON-TARGETplus Human RREB1 siRNA J-019150-05-0005, J-019150-07-0005, and J-019150-08-0005, referred to as siRREB1 #1, #3, and #4, respectively) (Dharmacon/Horizon Discovery), and control siRNA (siCT, Dharmacon/Horizon Discovery) were transfected using lipofectamine RNAiMAX (Thermo Fisher/ Invitrogen) (50 nM siRNA, lipofectamine ratio 1), using manufacturers protocols.

    Biomarker Discovery:

    Article Title: Targeted long-read sequencing of the Ewing sarcoma 6p25.1 susceptibility locus identifies germline-somatic interactions with EWSR1-FLI1 binding.
    Article Snippet: .. The A673 dCas9-KRAB-expressing cell line was created as described previously12; the A673 cell line was lentivirally transduced using the plasmid Lenti-dCas-KRAB-blast (Addgene) with a MOI of 3, cell selection was performed using 20 mg/mL blasticidin. siRNA transfection For qPCR knockdown validation following siRNA transfection, 75,000 A673 cells were seeded in each well of a 6-well plate. .. The following day, individual siRNAs against RREB1 (Dharmacon ON-TARGETplus Human RREB1 siRNA J-019150-05-0005, J-019150-07-0005, and J-019150-08-0005, referred to as siRREB1 #1, #3, and #4, respectively) (Dharmacon/Horizon Discovery), and control siRNA (siCT, Dharmacon/Horizon Discovery) were transfected using lipofectamine RNAiMAX (Thermo Fisher/ Invitrogen) (50 nM siRNA, lipofectamine ratio 1), using manufacturers protocols.

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